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Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.
Antibody dilution
1:154
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA054148
Antibody HPA059929
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human retina shows strong positivity in photoreceptor cells.
Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor cells and outer plexiform layer.
Expression
RNA: detected in 34 tissues Protein: detected in 14 cell types
RNA: detected in 34 tissues Protein: detected in 3 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1500
1:1000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA054148
Antibody HPA059929
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
98.3, 98.2, 66.5
98.3, 98.2
Antibody dilution
1:390
1:510
PROTEIN ARRAY
Antibody HPA054148
Antibody HPA059929
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3150
1:6150
ANTIGEN INFORMATION
Antibody HPA054148
Antibody HPA059929
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
51
57
Antigen sequence
DRDEIQLILPTRARLGKEPADCDEDELGEILKEELPGPTTFDIYEFHFSD
L
