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Immunohistochemical staining of human fallopian tube shows cytoplasmic positivity in ciliated glandular cells.
Immunohistochemical staining of human fallopian tube shows strong positivity in cilia.
Expression
RNA: detected in 11 tissues Protein: detected in 20 cell types
RNA: detected in 11 tissues Protein: detected in 8 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:32000
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038006
Antibody HPA038007
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410165)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410165)
Target mass (kDa)
22.1, 20.6
22.1, 20.6, 14.9
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA038006
Antibody HPA038007
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
