We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Antibody dilution
1:62
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA050942
Antibody CAB010420
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in seminiferous duct cells.
Expression
RNA: detected in 37 tissues Protein: detected in 70 cell types
RNA: detected in 37 tissues Protein: detected in 77 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:40
1:250
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA050942
Antibody CAB010420
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
23.4, 23.3, 19.8, 19.6, 15.1
23.4, 23.3, 19.8, 19.6, 15.1, 8.2
Antibody dilution
1:240
1:500
PROTEIN ARRAY
Antibody HPA050942
Antibody CAB010420
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
