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TRPM1

ANTIBODY INFORMATION
 
 

Antibody HPA014779

  

Antibody HPA014785

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA014779   HPA014785  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   4  

References

   3  

Proper citation

 Atlas Antibodies Cat#HPA014779, RRID:AB_2669090    

VALIDATION SUMMARY
 
 

Antibody HPA014779

  

Antibody HPA014785

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA014779

  

Antibody HPA014785

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.  

Antibody dilution

   1:50  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA014779

  

Antibody HPA014785

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human retina shows strong cytoplasmic positivity in inner nuclear layer and outer plexiform layer.    

Expression

 RNA: detected in 2 tissues
Protein: detected in 2 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:900    

Literature conformity

 Partly consistent with gene/protein characterization data.    

RNA consistency

 No internal RNA expression data available for correlation.    

WESTERN BLOT
 
 

Antibody HPA014779

  

Antibody HPA014785

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 186.5, 184.8, 182.2, 173.6, 158.4, 57.4   186.5, 184.8, 182.2, 173.6  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA014779

  

Antibody HPA014785

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA014779

  

Antibody HPA014785

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 113   119  

Antigen sequence

 
CEATYLLRQSSINSADGYSLYRYHFNGEELLFEDTSLSTSPGTGVRKKTC
SFRIKEEKDVKTHLVPECQNSLHLSLGTSTSATPDGSHLAVDDLKNAEES
KLGPDIGISKEDD
  
VGGVNQDVEYSSITDQQLTTEWQCQVQKITRSHSTDIPYIVSEAAVQAEH
KEQFADMQDEHHVAEAIPRIPRLSLTITDRNGMENLLSVKPDQTLGFPSL
RSKSLHGHPRNVKSIQGKL
 

Matching transcripts

 TRPM1-001 - ENSP00000380897 [100%]
TRPM1-002 - ENSP00000453477 [100%]
TRPM1-004 - ENSP00000256552 [100%]
TRPM1-007 - ENSP00000453119 [100%]
TRPM1-008 - ENSP00000452946 [100%]
TRPM1-201 - ENSP00000437849 [100%]
  TRPM1-001 - ENSP00000380897 [100%]
TRPM1-004 - ENSP00000256552 [100%]
TRPM1-007 - ENSP00000453119 [100%]
TRPM1-201 - ENSP00000437849 [100%]
 

Other gene match

    
ANTIGEN VIEW
TRPM1-001
TRPM1-002
TRPM1-004
TRPM1-007
TRPM1-008
TRPM1-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA014779

  

Antibody HPA014785

  

1.

   Autoantibodies in melanoma-associated retinopathy target TRPM1 cation channels of retinal ON bipolar cells
Dhingra A et al
J Neurosci 2011;31(11):3962-7
  

2.

   Serum TRPM1 autoantibodies from melanoma associated retinopathy patients enter retinal on-bipolar cells and attenuate the electroretinogram in mice
Xiong WH et al
PLoS One 2013;8(8):e69506
  

3.

   Alterations in Kainate Receptor and TRPM1 Localization in Bipolar Cells after Retinal Photoreceptor Degeneration
Gayet-Primo J et al
Front Cell Neurosci 2015;9:486
Application: IHC 		 
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.