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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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ROCK2
  • TISSUE

  • premium

    CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

ROCK2

Gene description

Rho-associated, coiled-coil containing protein kinase 2

Protein class

Enzymes
Plasma proteins
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

4
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic expression in most tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly not consistent with RNA expression data.

Reliability score

Approved based on 2 antibodies.
HPA007459 , CAB008666
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PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (unfavourable).
Renal cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA007459
CAB008666

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Colorectal cancers showed strong cytoplasmic staining. Cancer cells in general showed weak to moderate immunoreactivity. Several renal cancers were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancer tissues showed weak to moderate cytoplasmic staining. Lymphomas and most renal cancers were negative.

GENE INFORMATION

Gene name

ROCK2

Synonyms

Description

Rho-associated, coiled-coil containing protein kinase 2 (HGNC Symbol)

Entrez gene summary

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]

Chromosome

2

Cytoband

p25.1

Chromosome location (bp)

11179761 - 11348330

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000134318 (version 83.38)

Entrez gene

9475

UniProt

O75116 (UniProt - Evidence at protein level)

neXtProt

NX_O75116

Antibodypedia

ROCK2 antibodies

PROTEIN BROWSER

ROCK2-001
ROCK2-006
ROCK2-007
ROCK2-201

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

ROCK2-001
ENSP00000317985
ENST00000315872
O75116 [Direct mapping]
Rho-associated protein kinase 2
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Enzymes
   ENZYME proteins
   Transferases
   Kinases
   AGC Ser/Thr protein kinases
   MEMSAT-SVM predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000910 [cytokinesis]
GO:0001934 [positive regulation of protein phosphorylation]
GO:0004672 [protein kinase activity]
GO:0004674 [protein serine/threonine kinase activity]
GO:0005198 [structural molecule activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005813 [centrosome]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006468 [protein phosphorylation]
GO:0006939 [smooth muscle contraction]
GO:0007165 [signal transduction]
GO:0007249 [I-kappaB kinase/NF-kappaB signaling]
GO:0007264 [small GTPase mediated signal transduction]
GO:0007266 [Rho protein signal transduction]
GO:0007411 [axon guidance]
GO:0010595 [positive regulation of endothelial cell migration]
GO:0010628 [positive regulation of gene expression]
GO:0016525 [negative regulation of angiogenesis]
GO:0017048 [Rho GTPase binding]
GO:0017049 [GTP-Rho binding]
GO:0030036 [actin cytoskeleton organization]
GO:0030155 [regulation of cell adhesion]
GO:0030866 [cortical actin cytoskeleton organization]
GO:0032956 [regulation of actin cytoskeleton organization]
GO:0035509 [negative regulation of myosin-light-chain-phosphatase activity]
GO:0035556 [intracellular signal transduction]
GO:0036464 [cytoplasmic ribonucleoprotein granule]
GO:0042752 [regulation of circadian rhythm]
GO:0044822 [poly(A) RNA binding]
GO:0045616 [regulation of keratinocyte differentiation]
GO:0046872 [metal ion binding]
GO:0048010 [vascular endothelial growth factor receptor signaling pathway]
GO:0048013 [ephrin receptor signaling pathway]
GO:0048511 [rhythmic process]
GO:0051298 [centrosome duplication]
GO:0051492 [regulation of stress fiber assembly]
GO:0051893 [regulation of focal adhesion assembly]
GO:0071394 [cellular response to testosterone stimulus]
GO:0072518 [Rho-dependent protein serine/threonine kinase activity]
GO:0090002 [establishment of protein localization to plasma membrane]
GO:1903140 [regulation of establishment of endothelial barrier]
GO:1903347 [negative regulation of bicellular tight junction assembly]
GO:2000114 [regulation of establishment of cell polarity]
GO:2000145 [regulation of cell motility]
Show all
1388 aa
160.9 kDa
No 0
ROCK2-006
ENSP00000385509
ENST00000401753
E9PF63 [Direct mapping]
Non-specific serine/threonine protein kinase
Show all
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004672 [protein kinase activity]
GO:0004674 [protein serine/threonine kinase activity]
GO:0005524 [ATP binding]
GO:0005622 [intracellular]
GO:0006468 [protein phosphorylation]
GO:0007165 [signal transduction]
GO:0007266 [Rho protein signal transduction]
GO:0017048 [Rho GTPase binding]
GO:0017049 [GTP-Rho binding]
GO:0035556 [intracellular signal transduction]
GO:0046872 [metal ion binding]
GO:0051492 [regulation of stress fiber assembly]
GO:2000114 [regulation of establishment of cell polarity]
Show all
1145 aa
133.3 kDa
No 0
ROCK2-007
ENSP00000395957
ENST00000431087
C9JFJ0 [Direct mapping]
Rho-associated protein kinase 2
Show all
   MEMSAT-SVM predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004672 [protein kinase activity]
GO:0004674 [protein serine/threonine kinase activity]
GO:0005524 [ATP binding]
GO:0005622 [intracellular]
GO:0006468 [protein phosphorylation]
GO:0007266 [Rho protein signal transduction]
GO:0017049 [GTP-Rho binding]
GO:0051492 [regulation of stress fiber assembly]
GO:2000114 [regulation of establishment of cell polarity]
Show all
203 aa
23.2 kDa
No 0
ROCK2-201
ENSP00000481789
ENST00000616279
Q14DU5 [Direct mapping]
ROCK2 protein; Rho-associated protein kinase 2
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004674 [protein serine/threonine kinase activity]
GO:0005622 [intracellular]
GO:0006468 [protein phosphorylation]
GO:0007266 [Rho protein signal transduction]
GO:0017048 [Rho GTPase binding]
GO:0017049 [GTP-Rho binding]
GO:0035556 [intracellular signal transduction]
GO:0046872 [metal ion binding]
GO:0051492 [regulation of stress fiber assembly]
GO:2000114 [regulation of establishment of cell polarity]
Show all
703 aa
82.1 kDa
No 0

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