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ANTIBODY INFORMATION
 
 

Antibody HPA061250

  

Antibody HPA063127

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA061250   HPA063127  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA061250, RRID:AB_2684458   Atlas Antibodies Cat#HPA063127, RRID:AB_2684944  

VALIDATION SUMMARY
 
 

Antibody HPA061250

  

Antibody HPA063127

  
Immunocytochemistry

Approved    
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA061250

  

Antibody HPA063127

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.    

Antibody dilution

 1:79    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA061250

  

Antibody HPA063127

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human retina shows cytoplasmic positivity in all layers of retina.   Immunohistochemical staining of human retina shows moderate cytoplasmic positivity in phoreceptor cells and outer plexiform layer.  

Expression

 RNA: detected in 2 tissues
Protein: detected in 20 cell types		   RNA: detected in 2 tissues
Protein: detected in 28 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:500  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.  

WESTERN BLOT
 
 

Antibody HPA061250

  

Antibody HPA063127

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 154.2, 151.2, 142.1, 74.7   154.2, 151.2, 142.1, 74.7  

Antibody dilution

 1:260   1:80  

PROTEIN ARRAY
 
 

Antibody HPA061250

  

Antibody HPA063127

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3150   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA061250

  

Antibody HPA063127

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 79   96  

Antigen sequence

 
LLLALENSTYQYIRVWLERGRLAMLTPNSPKLVVKFVLNDGNVHLISLKI
KPYKIELYQSSQNLGFISASTWKIEKGDV
  
NHITLENISSGSSLNVKAGCVRKDWCESQPCQSRGRCINLWLSYQCDCHR
PYEGPNCLREYVAGRFGQDDSTGYVIFTLDESYGDTISLSMFVRTL
 

Matching transcripts

 CRB1-003 - ENSP00000356367 [100%]
CRB1-004 - ENSP00000356370 [100%]
CRB1-005 - ENSP00000356369 [100%]
CRB1-201 - ENSP00000438786 [100%]
  CRB1-003 - ENSP00000356367 [100%]
CRB1-004 - ENSP00000356370 [100%]
CRB1-005 - ENSP00000356369 [100%]
CRB1-201 - ENSP00000438786 [100%]
 

Other gene match

    
ANTIGEN VIEW
CRB1-003
CRB1-004
CRB1-005
CRB1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.