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ANTIBODY INFORMATION
 
 

Antibody HPA054720

  

Antibody HPA075003

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA054720   HPA075003  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA054720, RRID:AB_2682584   Atlas Antibodies Cat#HPA075003, RRID:AB_2686727  

VALIDATION SUMMARY
 
 

Antibody HPA054720

  

Antibody HPA075003

  
Immunocytochemistry


Validated
  

Validated
  
Western blot

Uncertain    
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA054720

  

Antibody HPA075003

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA075003.		  
Validated

Antibody staining overlaps with antibody HPA054720.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line AF22 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line AF22 shows localization to nucleoplasm.  

Antibody dilution

 1:9   1:47  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

WESTERN BLOT
 
 

Antibody HPA054720

  

Antibody HPA075003

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		    

   

Figure description

    

Target mass (kDa)

 45.2    

Antibody dilution

 1:90    

PROTEIN ARRAY
 
 

Antibody HPA054720

  

Antibody HPA075003

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:750   1:1900  

ANTIGEN INFORMATION
 
 

Antibody HPA054720

  

Antibody HPA075003

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 29   62  

Antigen sequence

 
TLAHLLPAPAMYSLLETELKNPVGTPTQA
  
RPVRENSSGARSPRVPADLARSILISLPFPPDSLAHRPPSSAPTESQGLF
TVAAPAPGAPSP
 

Matching transcripts

 SOX3-001 - ENSP00000359567 [100%]
  SOX3-001 - ENSP00000359567 [100%]
 

Other gene match

    
ANTIGEN VIEW
SOX3-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.