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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.
Antibody dilution
1:22
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA040596
Antibody HPA040617
Antibody HPA061753
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 70 cell types
RNA: detected in 37 tissues Protein: detected in 35 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:85
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040596
Antibody HPA040617
Antibody HPA061753
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414528)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
34.4, 33.3, 27.8, 26.1, 25.7, 23.5, 14.5
33.3, 29.6, 28.7, 19
34.4, 33.3, 29.6, 27.8, 26.1, 25.7, 23.5, 21.2
Antibody dilution
1:250
1:250
1:80
PROTEIN ARRAY
Antibody HPA040596
Antibody HPA040617
Antibody HPA061753
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
