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Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

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Score

Score

Score

Antibodies

Column

 
 
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P2RX4
  • TISSUE

  • CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

P2RX4

Gene description

Purinergic receptor P2X, ligand gated ion channel, 4

Protein class

Predicted membrane proteins
Transporters

Predicted localization

Membrane

Number of transcripts

5
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Mixed
HPA (normal tissue):Mixed

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic and luminal membrane expression in most tissues, highly abundant in placental trophoblasts, lung macrophages and gastrointestinal mucosa.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data. Pending external verification.

Reliability score

Approved based on 1 antibody.
HPA039494
SHOW MORE

PROGNOSTIC SUMMARY

Prognostic marker in endometrial cancer (favourable).
Endometrial cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA039494

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancer cells showed weak to moderate cytoplasmic staining with occasional membranous immunoreactivity. A few prostate, ovarian, endometrial and pancreatic cancers were strongly stained. Colorectal cancers displayed additional membranous staining. Most lymphomas and several gliomas were negative.

GENE INFORMATION

Gene name

P2RX4 (HGNC Symbol)

Synonyms

P2X4

Description

Purinergic receptor P2X, ligand gated ion channel, 4 (HGNC Symbol)

Entrez gene summary

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants, some protein-coding and some not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012]

Chromosome

12

Cytoband

q24.31

Chromosome location (bp)

121209857 - 121234106

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000135124 (version 83.38)

Entrez gene

5025

UniProt

Q99571 (UniProt - Evidence at protein level)

neXtProt

NX_Q99571

Antibodypedia

P2RX4 antibodies

PROTEIN BROWSER

P2RX4-001
P2RX4-002
P2RX4-005
P2RX4-011
P2RX4-201

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

P2RX4-001
ENSP00000336607
ENST00000337233
Q99571 [Direct mapping]
P2X purinoceptor 4
Show all
Transporters
   Transporter channels and pores
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   2TM proteins predicted by MDM
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001614 [purinergic nucleotide receptor activity]
GO:0001894 [tissue homeostasis]
GO:0002028 [regulation of sodium ion transport]
GO:0004931 [extracellular ATP-gated cation channel activity]
GO:0005102 [receptor binding]
GO:0005507 [copper ion binding]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005639 [integral component of nuclear inner membrane]
GO:0005765 [lysosomal membrane]
GO:0005886 [plasma membrane]
GO:0005887 [integral component of plasma membrane]
GO:0006810 [transport]
GO:0006812 [cation transport]
GO:0007165 [signal transduction]
GO:0008217 [regulation of blood pressure]
GO:0008270 [zinc ion binding]
GO:0010524 [positive regulation of calcium ion transport into cytosol]
GO:0010614 [negative regulation of cardiac muscle hypertrophy]
GO:0016020 [membrane]
GO:0019233 [sensory perception of pain]
GO:0030054 [cell junction]
GO:0032308 [positive regulation of prostaglandin secretion]
GO:0033198 [response to ATP]
GO:0034220 [ion transmembrane transport]
GO:0034405 [response to fluid shear stress]
GO:0035590 [purinergic nucleotide receptor signaling pathway]
GO:0042118 [endothelial cell activation]
GO:0042803 [protein homodimerization activity]
GO:0045296 [cadherin binding]
GO:0045429 [positive regulation of nitric oxide biosynthetic process]
GO:0048471 [perinuclear region of cytoplasm]
GO:0050850 [positive regulation of calcium-mediated signaling]
GO:0051899 [membrane depolarization]
GO:0051928 [positive regulation of calcium ion transport]
GO:0055117 [regulation of cardiac muscle contraction]
GO:0055119 [relaxation of cardiac muscle]
GO:0070062 [extracellular exosome]
GO:0071318 [cellular response to ATP]
GO:0097190 [apoptotic signaling pathway]
GO:0098655 [cation transmembrane transport]
Show all
388 aa
43.4 kDa
No 2
P2RX4-002
ENSP00000353032
ENST00000359949
Q99571 [Direct mapping]
P2X purinoceptor 4
Show all
Transporters
   Transporter channels and pores
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   2TM proteins predicted by MDM
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001614 [purinergic nucleotide receptor activity]
GO:0001894 [tissue homeostasis]
GO:0002028 [regulation of sodium ion transport]
GO:0004931 [extracellular ATP-gated cation channel activity]
GO:0005102 [receptor binding]
GO:0005507 [copper ion binding]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005639 [integral component of nuclear inner membrane]
GO:0005765 [lysosomal membrane]
GO:0005886 [plasma membrane]
GO:0005887 [integral component of plasma membrane]
GO:0006809 [nitric oxide biosynthetic process]
GO:0006810 [transport]
GO:0006812 [cation transport]
GO:0006816 [calcium ion transport]
GO:0007165 [signal transduction]
GO:0008144 [drug binding]
GO:0008217 [regulation of blood pressure]
GO:0008270 [zinc ion binding]
GO:0010524 [positive regulation of calcium ion transport into cytosol]
GO:0010614 [negative regulation of cardiac muscle hypertrophy]
GO:0014069 [postsynaptic density]
GO:0016020 [membrane]
GO:0019228 [neuronal action potential]
GO:0019233 [sensory perception of pain]
GO:0030054 [cell junction]
GO:0030424 [axon]
GO:0030425 [dendrite]
GO:0032308 [positive regulation of prostaglandin secretion]
GO:0033198 [response to ATP]
GO:0034220 [ion transmembrane transport]
GO:0034405 [response to fluid shear stress]
GO:0035590 [purinergic nucleotide receptor signaling pathway]
GO:0042118 [endothelial cell activation]
GO:0042311 [vasodilation]
GO:0042803 [protein homodimerization activity]
GO:0043025 [neuronal cell body]
GO:0043195 [terminal bouton]
GO:0043197 [dendritic spine]
GO:0045177 [apical part of cell]
GO:0045202 [synapse]
GO:0045296 [cadherin binding]
GO:0045429 [positive regulation of nitric oxide biosynthetic process]
GO:0048471 [perinuclear region of cytoplasm]
GO:0050850 [positive regulation of calcium-mediated signaling]
GO:0051260 [protein homooligomerization]
GO:0051899 [membrane depolarization]
GO:0051928 [positive regulation of calcium ion transport]
GO:0055117 [regulation of cardiac muscle contraction]
GO:0055119 [relaxation of cardiac muscle]
GO:0060079 [excitatory postsynaptic potential]
GO:0070062 [extracellular exosome]
GO:0071318 [cellular response to ATP]
GO:0097190 [apoptotic signaling pathway]
GO:0098655 [cation transmembrane transport]
Show all
404 aa
45.3 kDa
No 2
P2RX4-005
ENSP00000438329
ENST00000542067
Q99571 [Direct mapping]
P2X purinoceptor 4
Show all
Transporters
   Transporter channels and pores
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   2TM proteins predicted by MDM
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001614 [purinergic nucleotide receptor activity]
GO:0001894 [tissue homeostasis]
GO:0002028 [regulation of sodium ion transport]
GO:0004931 [extracellular ATP-gated cation channel activity]
GO:0005102 [receptor binding]
GO:0005507 [copper ion binding]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005639 [integral component of nuclear inner membrane]
GO:0005765 [lysosomal membrane]
GO:0005886 [plasma membrane]
GO:0005887 [integral component of plasma membrane]
GO:0006810 [transport]
GO:0006812 [cation transport]
GO:0007165 [signal transduction]
GO:0008217 [regulation of blood pressure]
GO:0008270 [zinc ion binding]
GO:0010524 [positive regulation of calcium ion transport into cytosol]
GO:0010614 [negative regulation of cardiac muscle hypertrophy]
GO:0016020 [membrane]
GO:0019233 [sensory perception of pain]
GO:0030054 [cell junction]
GO:0032308 [positive regulation of prostaglandin secretion]
GO:0033198 [response to ATP]
GO:0034220 [ion transmembrane transport]
GO:0034405 [response to fluid shear stress]
GO:0035590 [purinergic nucleotide receptor signaling pathway]
GO:0042118 [endothelial cell activation]
GO:0042803 [protein homodimerization activity]
GO:0045296 [cadherin binding]
GO:0045429 [positive regulation of nitric oxide biosynthetic process]
GO:0048471 [perinuclear region of cytoplasm]
GO:0050850 [positive regulation of calcium-mediated signaling]
GO:0051899 [membrane depolarization]
GO:0051928 [positive regulation of calcium ion transport]
GO:0055117 [regulation of cardiac muscle contraction]
GO:0055119 [relaxation of cardiac muscle]
GO:0070062 [extracellular exosome]
GO:0071318 [cellular response to ATP]
GO:0097190 [apoptotic signaling pathway]
GO:0098655 [cation transmembrane transport]
Show all
361 aa
40.5 kDa
No 2
P2RX4-011
ENSP00000444033
ENST00000538701
F5H1M6 [Direct mapping]
P2X purinoceptor 4
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001614 [purinergic nucleotide receptor activity]
GO:0004931 [extracellular ATP-gated cation channel activity]
GO:0005524 [ATP binding]
GO:0005639 [integral component of nuclear inner membrane]
GO:0005887 [integral component of plasma membrane]
GO:0006812 [cation transport]
GO:0033198 [response to ATP]
GO:0035590 [purinergic nucleotide receptor signaling pathway]
GO:0098655 [cation transmembrane transport]
Show all
145 aa
16.3 kDa
No 1
P2RX4-201
ENSP00000438131
ENST00000543171
F6SFZ6 [Direct mapping]
P2X purinoceptor 4
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001614 [purinergic nucleotide receptor activity]
GO:0004931 [extracellular ATP-gated cation channel activity]
GO:0005524 [ATP binding]
GO:0005639 [integral component of nuclear inner membrane]
GO:0005887 [integral component of plasma membrane]
GO:0006812 [cation transport]
GO:0033198 [response to ATP]
GO:0035590 [purinergic nucleotide receptor signaling pathway]
GO:0098655 [cation transmembrane transport]
Show all
341 aa
38 kDa
No 1

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