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AMHR2

ANTIBODY INFORMATION
 
 

Antibody HPA060228

  

Antibody HPA069636

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA060228   HPA069636  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA060228, RRID:AB_2684227   Atlas Antibodies Cat#HPA069636, RRID:AB_2686163  

VALIDATION SUMMARY
 
 

Antibody HPA060228

  

Antibody HPA069636

  
Immunocytochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA060228

  

Antibody HPA069636

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.   Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.  

Antibody dilution

 1:67   1:29  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA060228

  

Antibody HPA069636

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 62.7, 19.2   62.7, 52.2, 51.8  

Antibody dilution

 1:220   1:100  

PROTEIN ARRAY
 
 

Antibody HPA060228

  

Antibody HPA069636

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2650   1:1050  

ANTIGEN INFORMATION
 
 

Antibody HPA060228

  

Antibody HPA069636

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 95   87  

Antigen sequence

 
AGTQRYMAPELLDKTLDLQDWGMALRRADIYSLALLLWEILSRCPDLRPD
SSPPPFQLAYEAELGNTPTSDELWALAVQERRRPYIPSTWRCFAT
  
LHELCFSQVIREGGHAVVWAGQLQGKLVAIKAFPPRSVAQFQAERALYEL
PGLQHDHIVRFITASRGGPGRLLSGPLLVLELHPKGS
 

Matching transcripts

 AMHR2-001 - ENSP00000257863 [100%]
AMHR2-007 - ENSP00000455338 [100%]
  AMHR2-001 - ENSP00000257863 [99%]
AMHR2-002 - ENSP00000369117 [99%]
AMHR2-003 - ENSP00000446661 [99%]
 

Other gene match

    
ANTIGEN VIEW
AMHR2-001
AMHR2-002
AMHR2-003
AMHR2-007
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.