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Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in subsets of germinal center cells.
Immunohistochemical staining of human tonsil shows distinct cytoplasmic positivity in subsets of cells.
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in subsets of germinal center cells.
Expression
RNA: detected in 36 tissues Protein: detected in 53 cell types
RNA: detected in 36 tissues Protein: detected in 64 cell types
RNA: detected in 36 tissues Protein: detected in 16 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:600
1:600
1:200
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030175
Antibody HPA030176
Antibody HPA030178
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402940)
Target mass (kDa)
119.8, 117.9, 108.6
119.8, 117.9, 108.6
119.8, 117.9, 108.6, 27.1
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA030175
Antibody HPA030176
Antibody HPA030178
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
