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ANTIBODY INFORMATION
 
 

Antibody HPA007316

  

Antibody HPA007571

  

Antibody CAB008636

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   AbFrontier
  

Product name

 HPA007316   HPA007571   LF-PA0100  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Ammonium Sulfate Precipitation  

Other gene match

      

Released in version

 3   3   3  

References

 2   1    

Proper citation

 Atlas Antibodies Cat#HPA007316, RRID:AB_888438   Atlas Antibodies Cat#HPA007571, RRID:AB_1078980   ABFrontier Cat#LF-PA0100, RRID:AB_1616669  

VALIDATION SUMMARY
 
 

Antibody HPA007316

  

Antibody HPA007571

  

Antibody CAB008636

  
Immunocytochemistry

  Supported   Supported  
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA007316

  

Antibody HPA007571

  

Antibody CAB008636

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & mitochondria.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, cytosol & mitochondria.  

Antibody dilution

   1:5   1:188  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007316

  

Antibody HPA007571

  

Antibody CAB008636

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in subsets of glial cells.   Immunohistochemical staining of human hippocampus shows distinct positivity in astrocyte like cells.   Immunohistochemical staining of human cerebral cortex shows distinct cytoplasmic and nuclear positivity in glial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 35 cell types		   RNA: detected in 37 tissues
Protein: detected in 46 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:10   1:750  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007316

  

Antibody HPA007571

  

Antibody CAB008636

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400756)		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400756)		    

Target mass (kDa)

 42.1   42.1   42.1, 26.4  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA007316

  

Antibody HPA007571

  

Antibody CAB008636

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA007316

  

Antibody HPA007571

  

Antibody CAB008636

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 141   139    

Antigen sequence

 
LQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTC
KRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGA
DRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGP
  
RVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEK
LSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIP
RTVGQEKKGYFEDRRPSANCDPFSVTEALIRTCLLNETG
  

											 
																				

																		

										
											
Matching transcripts

												
																					GLUL-001 - ENSP00000356537 [100%] 
GLUL-002 - ENSP00000344958 [100%] 
GLUL-201 - ENSP00000307900 [100%] 
GLUL-202 - ENSP00000398320 [100%] 

											 
																						GLUL-001 - ENSP00000356537 [100%] 
GLUL-002 - ENSP00000344958 [100%] 
GLUL-201 - ENSP00000307900 [100%] 
GLUL-202 - ENSP00000398320 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
GLUL-001
GLUL-002
GLUL-201
GLUL-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA007316

												
										 
																				
											
											
											
Antibody HPA007571

												
										 
																				
											
											
											
Antibody CAB008636

												
										 
																		

																	

										
											
1.

												
																						Glutamine fuels a vicious cycle of autophagy in the tumor stroma and oxidative mitochondrial metabolism in epithelial cancer cells: implications for preventing chemotherapy resistance

	Ko YH et al

	Cancer Biol Ther 2011;12(12):1085-97

			
											 
																							Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

	Stadler C et al

	Nat Methods 2013;10(4):315-23

			
											 
																						
											 
																				

																		

										
											
2.

												
																						Profiling of atherosclerotic lesions by gene and tissue microarrays reveals PCSK6 as a novel protease in unstable carotid atherosclerosis

	Perisic L et al

	Arterioscler Thromb Vasc Biol 2013;33(10):2432-43

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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