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Antibody staining overlaps with antibody HPA063658.
Validated
Antibody staining overlaps with antibody HPA012867.
Standard validation
Approved
Approved
Figure description
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.
Antibody dilution
1:45
1:139
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA012867
Antibody HPA063658
Antibody CAB011448
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human endometrium shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human endometrium shows cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 22 tissues Protein: detected in 29 cell types
RNA: detected in 22 tissues Protein: detected in 68 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:700
1:1100
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA012867
Antibody HPA063658
Antibody CAB011448
Standard validation
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400312)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
54.3
54.3
54.3
Antibody dilution
1:250
1:460
1:500
PROTEIN ARRAY
Antibody HPA012867
Antibody HPA063658
Antibody CAB011448
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
