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USP44

ANTIBODY INFORMATION
 
 

Antibody HPA026543

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA026543  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 5  

References

  

Proper citation

  

VALIDATION SUMMARY
 
 

Antibody HPA026543

  
Immunocytochemistry

Uncertain  
Western blot

Supported  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA026543

  

Standard validation

 Uncertain  

 

Figure description

 Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoli fibrillar center & microtubules.  

Antibody dilution

 1:30  

Literature conformity

 The subcellular location is not consistent with literature.  

WESTERN BLOT
 
 

Antibody HPA026543

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

 

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 81.2, 56.5  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA026543

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA026543

  

Antigen

 Recombinant protein fragment  

Length (aa)

 146  

Antigen sequence

 
AIKSQNYHCTTRSGRFLRSMGTGDDSYFLHDGAQSLLQSEDQLYTALWHR
RRILMGKIFRTWFEQSPIGRKKQEEPFQEKIVVKREVKKRRQELEYQVKA
ELESMPPRKSLRLQGLAQSTIIEIVSVQVPAQTPASPAKDKVLSTS
 

Matching transcripts

 USP44-001 - ENSP00000258499 [100%]
USP44-002 - ENSP00000442629 [100%]
USP44-003 - ENSP00000448670 [100%]
USP44-004 - ENSP00000376806 [100%]
 

Other gene match

  
ANTIGEN VIEW
USP44-001
USP44-002
USP44-003
USP44-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.