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CKAP4

ANTIBODY INFORMATION
 
 

Antibody HPA000278

  

Antibody HPA000792

  

Antibody HPA001225

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA000278   HPA000792   HPA001225  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 1   2   12  

References

 1      

Proper citation

 Atlas Antibodies Cat#HPA000278, RRID:AB_1078527   Atlas Antibodies Cat#HPA000792, RRID:AB_1078526   Atlas Antibodies Cat#HPA001225, RRID:AB_2666241  

VALIDATION SUMMARY
 
 

Antibody HPA000278

  

Antibody HPA000792

  

Antibody HPA001225

  
Immunocytochemistry

    Supported  
Immunohistochemistry

Supported   Supported    
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000278

  

Antibody HPA000792

  

Antibody HPA001225

  

Standard validation

     Supported  

     

Figure description

     Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles & cytosol.  

Antibody dilution

     1:20  

Literature conformity

     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000278

  

Antibody HPA000792

  

Antibody HPA001225

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 56 cell types		   RNA: detected in 37 tissues
Protein: detected in 65 cell types		    

Retrieval

 None   HIER pH6    

Antibody dilution

 1:100   1:750    

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA000278

  

Antibody HPA000792

  

Antibody HPA001225

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 66   66   66  

Antibody dilution

 1:500   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA000278

  

Antibody HPA000792

  

Antibody HPA001225

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1000   1:1000   1:1000  

ANTIGEN INFORMATION
 
 

Antibody HPA000278

  

Antibody HPA000792

  

Antibody HPA001225

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 110   108   108  

Antigen sequence

 
SRLQHVEDGVLSMQVASARQTESLESLLSKSQEHEQRLAALQGRLEGLGS
SEADQDGLASTVRSLGETQLVLYGDVEELKRSVGELPSTVESLQKVQEQV
HTLLSQDQAQ
  
LKDLSDGIHVVKDARERDFTSLENTVEERLTELTKSINDNIAIFTEVQKR
SQKEINDMKAKVASLEESEGNKQDLKALKEAVKEIQTSAKSREWDMEALR
STLQTMES
  
LKDLSDGIHVVKDARERDFTSLENTVEERLTELTKSINDNIAIFTEVQKR
SQKEINDMKAKVASLEESEGNKQDLKALKEAVKEIQTSAKSREWDMEALR
STLQTMES
 

Matching transcripts

 CKAP4-001 - ENSP00000367265 [100%]
  CKAP4-001 - ENSP00000367265 [100%]
  CKAP4-001 - ENSP00000367265 [100%]
 

Other gene match

      
ANTIGEN VIEW
CKAP4-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA000278

  

Antibody HPA000792

  

Antibody HPA001225

  

1.

 From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies
Ek S et al
Mol Cell Proteomics 2006;5(6):1072-81
      
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.