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RAC1

ANTIBODY INFORMATION
 
 

Antibody HPA047820

  

Antibody CAB035994

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   NCI-CPTC
  

Product name

 HPA047820   CPTC-RAC1-1  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

 RAC2 - ENSG00000128340 [86%]
RAC3 - ENSG00000169750 [100%]
    

Released in version

 12   7  

References

    

Proper citation

 Atlas Antibodies Cat#HPA047820, RRID:AB_2680167    

VALIDATION SUMMARY
 
 

Antibody HPA047820

  

Antibody CAB035994

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Supported  
Protein array

Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA047820

  

Antibody CAB035994

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to nucleus, nucleoli & cytosol.  

Antibody dilution

   1:20  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047820

  

Antibody CAB035994

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.   Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 41 cell types		   RNA: detected in 37 tissues
Protein: detected in 34 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:35  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA047820

  

Antibody CAB035994

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 23.5, 21.4, 20.8, 18.5, 16.8, 15.4, 14.8   23.5, 21.4  

Antibody dilution

 1:230   1:500  

PROTEIN ARRAY
 
 

Antibody HPA047820

  

Antibody CAB035994

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1800    

ANTIGEN INFORMATION
 
 

Antibody HPA047820

  

Antibody CAB035994

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 35    

Antigen sequence

 
TKLDLRDDKDTIERLRDKKLAPITYPQGLAMAREI
   

Matching transcripts

 RAC1-001 - ENSP00000258737 [86%]
RAC1-002 - ENSP00000348461 [86%]
   

Other gene match

 RAC2 - ENSG00000128340 [86%]
RAC3 - ENSG00000169750 [100%]
    
ANTIGEN VIEW
RAC1-001
RAC1-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.