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TTC5

ANTIBODY INFORMATION
 
 

Antibody HPA045008

  

Antibody HPA054198

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA045008   HPA054198  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   12  

References

    

Proper citation

 Atlas Antibodies Cat#HPA045008, RRID:AB_10960209   Atlas Antibodies Cat#HPA054198, RRID:AB_2682414  

VALIDATION SUMMARY
 
 

Antibody HPA045008

  

Antibody HPA054198

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA045008

  

Antibody HPA054198

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.  

Antibody dilution

   1:38  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045008

  

Antibody HPA054198

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 33 tissues
Protein: detected in 50 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:50    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA045008

  

Antibody HPA054198

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 48.9   48.9  

Antibody dilution

 1:250   1:190  

PROTEIN ARRAY
 
 

Antibody HPA045008

  

Antibody HPA054198

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:1550  

ANTIGEN INFORMATION
 
 

Antibody HPA045008

  

Antibody HPA054198

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 99   86  

Antigen sequence

 
GALTHCRNKVSLQNLSMVLRQLRTDTEDEHSHHVMDSVRQAKLAVQMDVH
DGRSWYILGNSYLSLYFSTGQNPKISQQALSAYAQAEKVDRKASSNPDL
  
TFGLVDSDGPCYAVMVYNIVQSWGVLIGDSVAIPEPNLRLHRIQHKGKDY
SFSSVRVETPLLLVVNGKPQGSSSQAVATVASRPQC
 

Matching transcripts

 TTC5-001 - ENSP00000258821 [100%]
  TTC5-001 - ENSP00000258821 [100%]
 

Other gene match

    
ANTIGEN VIEW
TTC5-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.