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TLR4

ANTIBODY INFORMATION
 
 

Antibody HPA049174

  

Antibody CAB004025

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Cell Signaling Technology, Inc
  

Product name

 HPA049174   2246  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 13   2  

References

    

Proper citation

 Atlas Antibodies Cat#HPA049174, RRID:AB_2680662    

VALIDATION SUMMARY
 
 

Antibody HPA049174

  

Antibody CAB004025

  
Immunocytochemistry

Uncertain    
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Supported  
Protein array

Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA049174

  

Antibody CAB004025

  

Standard validation

 Uncertain    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & the Golgi apparatus.    

Antibody dilution

 1:9    

Literature conformity

 The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA049174

  

Antibody CAB004025

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human spleen shows distinct cytoplasmic positivity in subsets of cells in the red pulp.   Immunohistochemical staining of human spleen shows distinct cytoplasmic positivity in subsets of cells in the red pulp.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 16 cell types		   RNA: detected in 35 tissues
Protein: detected in 36 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:175   1:175  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA049174

  

Antibody CAB004025

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Testis		  

Target mass (kDa)

 95.7, 91.3   95.7, 91.3  

Antibody dilution

 1:90   1:500  

PROTEIN ARRAY
 
 

Antibody HPA049174

  

Antibody CAB004025

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:700    

ANTIGEN INFORMATION
 
 

Antibody HPA049174

  

Antibody CAB004025

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 91    

Antigen sequence

 
LQVLNMSHNNFFSLDTFPYKCLNSLQVLDYSLNHIMTSKKQELQHFPSSL
AFLNLTQNDFACTCEHQSFLQWIKDQRQLLVEVERMECATP
   

Matching transcripts

 TLR4-001 - ENSP00000363089 [100%]
TLR4-002 - ENSP00000377997 [100%]
   

Other gene match

    
ANTIGEN VIEW
TLR4-001
TLR4-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.