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Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Antibody dilution
1:100
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA021251
Antibody HPA021330
Standard validation
Supported
Figure description
Immunohistochemical staining of human liver shows cytoplasmic positivity in hepatocytes.
Expression
RNA: detected in 2 tissues Protein: detected in 10 cell types
Retrieval
HIER pH6
Antibody dilution
1:625
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA021251
Antibody HPA021330
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419801)
Target mass (kDa)
46.3
46.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA021251
Antibody HPA021330
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
