TMEM63B - Antibodies - The Human Protein Atlas

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TMEM63B

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA029249

Antibody HPA029250

Antibody HPA029251

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA029249

HPA029250

HPA029251

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA029249, RRID:AB_2672965

Atlas Antibodies Cat#HPA029250, RRID:AB_2672966

Atlas Antibodies Cat#HPA029251, RRID:AB_2672967

VALIDATION SUMMARY

Antibody HPA029249

Antibody HPA029250

Antibody HPA029251

Immunocytochemistry

Immunohistochemistry

Uncertain

Western blot

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA029249

Antibody HPA029250

Antibody HPA029251

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA029251.

Validated

Antibody staining overlaps with antibody HPA029250.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & actin filaments.

Antibody dilution

1:43

1:7

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA029249

Antibody HPA029250

Antibody HPA029251

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.

Immunohistochemical staining of human kidney shows strong membranous positivity in tubular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 14 cell types

RNA: detected in 37 tissues
Protein: detected in 26 cell types

Retrieval

HIER pH6

Antibody dilution

1:25

1:30

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA029249

Antibody HPA029250

Antibody HPA029251

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

95, 86.7

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA029249

Antibody HPA029250

Antibody HPA029251

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA029249

Antibody HPA029250

Antibody HPA029251

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

RTLFINGISKYAESEKIKKHFEEAYPNCTVLEARPCYNVARLMFLDAERK
KAERGKLYFTN

KYLSAHNYKIEHTETDTVDPRSNGRPPTAAAVPKSAKYIAQVLQDSEVDG
DGDGAPGSSGDEPPSSSSQDEELLMPPD

SKENVPTMINPKPCGHLCCCVVRGCEQVEAIEYYTKLEQKLKEDYKREKE
KVNEKPLGMAFVTFHNETITAIILKDFNVCKCQGC

Matching transcripts

TMEM63B-004 - ENSP00000360960 [100%]
TMEM63B-005 - ENSP00000259746 [100%]
TMEM63B-201 - ENSP00000327154 [100%]

Other gene match

ANTIGEN VIEW

TMEM63B-004	TMEM63B-005	TMEM63B-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.