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Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Antibody dilution
1:13
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA003056
Antibody CAB002685
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human urinary bladder shows strong nuclear and cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 71 cell types
RNA: detected in 36 tissues Protein: detected in 52 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:500
1:50
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003056
Antibody CAB002685
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409612)
Target mass (kDa)
45.2, 42.9, 40.9, 35, 26.7
45.2, 42.9, 40.9, 35, 33, 29.8, 26.7, 10.4
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA003056
Antibody CAB002685
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
