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Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & microtubules.
Antibody dilution
1:400
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA027347
Antibody HPA027755
Antibody CAB022094
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 47 cell types
RNA: detected in 37 tissues Protein: detected in 71 cell types
RNA: detected in 37 tissues Protein: detected in 73 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:400
1:10
1:500
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027347
Antibody HPA027755
Antibody CAB022094
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408026)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
50.1, 49, 46.1, 29.2
50.1, 49, 46.1, 20.1, 14.1
50.1, 49, 46.1, 29.2, 20.1, 14.1, 5.6
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA027347
Antibody HPA027755
Antibody CAB022094
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
