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Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.
Antibody dilution
1:7
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA059756
Antibody CAB033678
Standard validation
Approved
Figure description
Immunohistochemical staining of human small intestine shows distinct membranous positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 58 cell types
Retrieval
HIER pH6
Antibody dilution
1:5000
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA059756
Antibody CAB033678
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
129.4
129.4, 10.1
Antibody dilution
1:80
1:500
PROTEIN ARRAY
Antibody HPA059756
Antibody CAB033678
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
