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LBX1

ANTIBODY INFORMATION
 
 

Antibody HPA002016

  

Antibody HPA002086

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA002016   HPA002086  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   3  

References

    

Proper citation

 Atlas Antibodies Cat#HPA002016, RRID:AB_2666518    

VALIDATION SUMMARY
 
 

Antibody HPA002016

  

Antibody HPA002086

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Uncertain  
Western blot

  Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002016

  

Antibody HPA002086

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm, nuclear bodies & cytosol.    

Antibody dilution

 1:5    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002016

  

Antibody HPA002086

  

Standard validation

   Uncertain  

   

Figure description

   Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells and Islet cells.  

Expression

   RNA: detected in 1 tissues
Protein: detected in 64 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:25  

Literature conformity

   No avaliable gene/protein characterization data.  

RNA consistency

   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002016

  

Antibody HPA002086

  

Standard validation

   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

   30.2  

Antibody dilution

   1:250  

PROTEIN ARRAY
 
 

Antibody HPA002016

  

Antibody HPA002086

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA002016

  

Antibody HPA002086

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 111   126  

Antigen sequence

 
ITWFQNRRAKLKRDLEEMKADVESAKKLGPSGQMDIVALAELEQNSEATA
GGGGGCGRAKSRPGSPVLPPGAPKAPGAGALQLSPASPLTDQPASSQDCS
EDEEDEEIDVD
  
PANSNKPLTPFSIEDILNKPSVRRSYSLCGAAHLLAAADKHAQGGLPLAG
RALLSQTSPLCALEELASKTFKGLEVSVLQAAEGRDGMTIFGQRQTPKKR
RKSRTAFTNHQIYELEKRFLYQKYLS
 

Matching transcripts

 LBX1-001 - ENSP00000359212 [100%]
  LBX1-001 - ENSP00000359212 [100%]
 

Other gene match

    
ANTIGEN VIEW
LBX1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.