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ZNF365

ANTIBODY INFORMATION
 
 

Antibody HPA052446

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA052446  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 11  

References

  

Proper citation

 Atlas Antibodies Cat#HPA052446, RRID:AB_2681836  

VALIDATION SUMMARY
 
 

Antibody HPA052446

  
Immunocytochemistry

Supported  
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA052446

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to microtubule organizing center & vesicles.  

Antibody dilution

 1:41  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA052446

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in subsets of glial cells.  

Expression

 RNA: detected in 17 tissues
Protein: detected in 5 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:900  

Literature conformity

 Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA052446

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 52.5, 46.5, 38.2  

Antibody dilution

 1:210  

PROTEIN ARRAY
 
 

Antibody HPA052446

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1650  

ANTIGEN INFORMATION
 
 

Antibody HPA052446

  

Antigen

 Recombinant protein fragment  

Length (aa)

 87  

Antigen sequence

 
RSGPGLPTSDTKASFEAHVREKFNRMVEAVDRTIEKRIDKLTKELAQKTA
ELLEVRAAFVQLTQKKQEVQRRERALNRQVDVAVEMI
 

Matching transcripts

 ZNF365-001 - ENSP00000378674 [100%]
ZNF365-003 - ENSP00000387091 [100%]
ZNF365-004 - ENSP00000378675 [100%]
 

Other gene match

  
ANTIGEN VIEW
ZNF365-001
ZNF365-003
ZNF365-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.