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ASNSD1

ANTIBODY INFORMATION
 
 

Antibody HPA036132

  

Antibody HPA036133

  

Antibody HPA075042

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036132   HPA036133   HPA075042  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 13   7   16  

References

      

Proper citation

 Atlas Antibodies Cat#HPA036132, RRID:AB_2674960   Atlas Antibodies Cat#HPA036133, RRID:AB_10696650   Atlas Antibodies Cat#HPA075042, RRID:AB_2686731  

VALIDATION SUMMARY
 
 

Antibody HPA036132

  

Antibody HPA036133

  

Antibody HPA075042

  
Immunocytochemistry

    Approved  
Immunohistochemistry

Uncertain   Uncertain    
Western blot

Uncertain   Supported    
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036132

  

Antibody HPA036133

  

Antibody HPA075042

  

Standard validation

     Approved  

     

Figure description

     Immunofluorescent staining of human cell line BJ shows localization to cytosol.  

Antibody dilution

     1:115  

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036132

  

Antibody HPA036133

  

Antibody HPA075042

  

Standard validation

 Uncertain   Uncertain    

     

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity with granular pattern in renal tubules.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 71 cell types		   RNA: detected in 37 tissues
Protein: detected in 67 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:175   1:75    

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA036132

  

Antibody HPA036133

  

Antibody HPA075042

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		    

     

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 72.1, 69.3   72.1, 69.3    

Antibody dilution

 1:250   1:250    

PROTEIN ARRAY
 
 

Antibody HPA036132

  

Antibody HPA036133

  

Antibody HPA075042

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:4600  

ANTIGEN INFORMATION
 
 

Antibody HPA036132

  

Antibody HPA036133

  

Antibody HPA075042

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 98   97   83  

Antigen sequence

 
ILQVFLTDVHMKEVIQQFIDVLSVAVKKRVLCLPRDENLTANEVLKTCDR
KANVAILFSGGIDSMVIATLADRHIPLDEPIDLLNVAFIAEEKTMPTT
  
NQWQEVPASGLFRIDLKSTVISRCIILQLYPWKYISRENIIEENVNSLSQ
ISADLPAFVSVVANEAKLYLEKPVVPLNMMLPQAALETHCSNISNVP
  
KLRRTRICHLIRPLDTVLDDSIGCAVWFASRGIGWLVAQEGVKSYQSNAK
VVLTGIGADEQLAGYSRHRVRFQSHGLEGLNKE
 

Matching transcripts

 ASNSD1-001 - ENSP00000260952 [100%]
ASNSD1-002 - ENSP00000406790 [100%]
  ASNSD1-001 - ENSP00000260952 [99%]
ASNSD1-002 - ENSP00000406790 [99%]
  ASNSD1-001 - ENSP00000260952 [100%]
ASNSD1-002 - ENSP00000406790 [100%]
ASNSD1-007 - ENSP00000476091 [100%]
 

Other gene match

      
ANTIGEN VIEW
ASNSD1-001
ASNSD1-002
ASNSD1-007
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.