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FAM13A

ANTIBODY INFORMATION
 
 

Antibody HPA038108

  

Antibody HPA038109

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA038108   HPA038109  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 13   7  

References

   2  

Proper citation

 Atlas Antibodies Cat#HPA038108, RRID:AB_2675837   Atlas Antibodies Cat#HPA038109, RRID:AB_10672619  

VALIDATION SUMMARY
 
 

Antibody HPA038108

  

Antibody HPA038109

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Supported  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038108

  

Antibody HPA038109

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli, cytosol & cell junctions.  

Antibody dilution

 1:41   1:125  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038108

  

Antibody HPA038109

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human duodenum shows nuclear positivity in glandular cells.   Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		   RNA: detected in 37 tissues
Protein: detected in 77 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:700   1:850  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038108

  

Antibody HPA038109

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 116.9, 80.2, 78.5, 77.2, 77.1   116.9, 80.2, 78.5, 77.2, 77.1, 71  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA038108

  

Antibody HPA038109

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA038108

  

Antibody HPA038109

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 72   70  

Antigen sequence

 
ESGTLSASSATSARQRRRQSKEQDEVRHGRDKGLINKENTPSGFNHLDDC
ILNTQEVEKVHKNTFGCAGERS
  
TDFSARCFLDQFEDDADGFISPMDDKIPSKCSQDTGLSNLHAASIPELLE
HLQEMREEKKRIRKKLRDFE
 

Matching transcripts

 FAM13A-001 - ENSP00000378450 [100%]
FAM13A-005 - ENSP00000423252 [100%]
FAM13A-006 - ENSP00000427189 [100%]
FAM13A-022 - ENSP00000264344 [100%]
FAM13A-024 - ENSP00000421562 [100%]
  FAM13A-001 - ENSP00000378450 [100%]
FAM13A-005 - ENSP00000423252 [100%]
FAM13A-006 - ENSP00000427189 [100%]
FAM13A-022 - ENSP00000264344 [100%]
FAM13A-023 - ENSP00000421914 [100%]
FAM13A-024 - ENSP00000421562 [100%]
 

Other gene match

    
ANTIGEN VIEW
FAM13A-001
FAM13A-005
FAM13A-006
FAM13A-022
FAM13A-023
FAM13A-024

RELEVANT PUBLICATIONS
 
 

Antibody HPA038108

  

Antibody HPA038109

  

1.

   Regulation of nuclear-cytoplasmic shuttling and function of Family with sequence similarity 13, member A (Fam13a), by B56-containing PP2As and Akt
Jin Z et al
Mol Biol Cell 2015;26(6):1160-73
  

2.

   Genes associated with polymorphic variants predicting lung function are differentially expressed during human lung development
Miller S et al
Respir Res 2016;17(1):95
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.