LARP1B - Antibodies - The Human Protein Atlas

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LARP1B

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA036280

Antibody HPA036281

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA036280

HPA036281

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA036280, RRID:AB_10673126

Atlas Antibodies Cat#HPA036281, RRID:AB_2675036

VALIDATION SUMMARY

Antibody HPA036280

Antibody HPA036281

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA036280

Antibody HPA036281

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies & cytosol.

Antibody dilution

1:133

1:200

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA036280

Antibody HPA036281

Standard validation

Approved

Figure description

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in seminiferous tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 32 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA036280

Antibody HPA036281

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

105.3, 59.5, 56.1, 41.4, 38.8

105.3, 65.7, 59.5, 41.4, 38.8

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA036280

Antibody HPA036281

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA036280

Antibody HPA036281

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

KHKWVPLHLDVVRSESQERPGSRNSSRCQPEANKPTHNNRRNDTRSWKRD
REKRDDQDDVSSVRSEGGNIRGSF

MENWPTPSELVNTGFQSVLSQGNKKPQNRKEKEEKVEKRSNSDSKENRET
KLNGPGENVSEDEAQSSNQRKRANK

Matching transcripts

LARP1B-001 - ENSP00000321997 [100%]
LARP1B-003 - ENSP00000377829 [100%]
LARP1B-004 - ENSP00000390395 [100%]
LARP1B-005 - ENSP00000422850 [100%]
LARP1B-006 - ENSP00000424383 [100%]

LARP1B-001 - ENSP00000321997 [100%]
LARP1B-003 - ENSP00000377829 [100%]
LARP1B-004 - ENSP00000390395 [100%]
LARP1B-005 - ENSP00000422850 [100%]
LARP1B-002 - ENSP00000427281 [97%]

Other gene match

ANTIGEN VIEW

LARP1B-001	LARP1B-002	LARP1B-003	LARP1B-004	LARP1B-005	LARP1B-006

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.