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ANXA3

ANTIBODY INFORMATION
 
 

Antibody HPA013398

  

Antibody HPA013431

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA013398   HPA013431  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   4  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA013398, RRID:AB_1844861   Atlas Antibodies Cat#HPA013431, RRID:AB_1844862  

VALIDATION SUMMARY
 
 

Antibody HPA013398

  

Antibody HPA013431

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry


Validated
  

Validated

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA013398

  

Antibody HPA013431

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA013431.		  
Validated

Antibody staining overlaps with antibody HPA013398.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.  

Antibody dilution

 1:61   1:56  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA013398

  

Antibody HPA013431

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA013431 across 75 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA013398 across 75 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules and Bowmans capsule.   Immunohistochemical staining of human kidney shows strong positivity.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 46 cell types		   RNA: detected in 37 tissues
Protein: detected in 70 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1250   1:400  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA013398

  

Antibody HPA013431

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 36.4, 32.1   36.4, 17.1, 14.9, 11.6  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA013398

  

Antibody HPA013431

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA013398

  

Antibody HPA013431

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 141   104  

Antigen sequence

 
ISQAYYTVYKKSLGDDISSETSGDFRKALLTLADGRRDESLKVDEHLAKQ
DAQILYKAGENRWGTDEDKFTEILCLRSFPQLKLTFDEYRNISQKDIVDS
IKGELSGHFEDLLLAIVNCVRNTPAFLAERLHRALKGIGTD
  
ASIWVGHRGTVRDYPDFSPSVDAEAIQKAIRGIGTDEKMLISILTERSNA
QRQLIVKEYQAAYGKELKDDLKGDLSGHFEHLMVALVTPPAVFDAKQLKK
SMKG
 

Matching transcripts

 ANXA3-001 - ENSP00000264908 [100%]
ANXA3-002 - ENSP00000423068 [100%]
ANXA3-003 - ENSP00000421015 [100%]
  ANXA3-001 - ENSP00000264908 [100%]
ANXA3-006 - ENSP00000421512 [100%]
ANXA3-007 - ENSP00000422281 [100%]
ANXA3-005 - ENSP00000424584 [99%]
 

Other gene match

    
ANTIGEN VIEW
ANXA3-001
ANXA3-002
ANXA3-003
ANXA3-005
ANXA3-006
ANXA3-007

RELEVANT PUBLICATIONS
 
 

Antibody HPA013398

  

Antibody HPA013431

  

1.

 Differential protein expression profiles of cyst fluid from papillary thyroid carcinoma and benign thyroid lesions
Dinets A et al
PLoS One 2015;10(5):e0126472
Application: IHC, WB 		   
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.