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GABARAPL1

ANTIBODY INFORMATION
 
 

Antibody HPA051386

  

Antibody HPA065883

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA051386   HPA065883  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

   GABARAP - ENSG00000170296 [96%]
  

Released in version

 10   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA051386, RRID:AB_2681463   Atlas Antibodies Cat#HPA065883, RRID:AB_2685574  

VALIDATION SUMMARY
 
 

Antibody HPA051386

  

Antibody HPA065883

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051386

  

Antibody HPA065883

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.  

Antibody dilution

   1:52  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051386

  

Antibody HPA065883

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human stomach, upper shows moderate cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 49 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:50    

Literature conformity

 Partly consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA051386

  

Antibody HPA065883

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 17   17, 14, 13.9, 11.7  

Antibody dilution

 1:320   1:110  

PROTEIN ARRAY
 
 

Antibody HPA051386

  

Antibody HPA065883

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:15000   1:2100  

ANTIGEN INFORMATION
 
 

Antibody HPA051386

  

Antibody HPA065883

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 57   25  

Antigen sequence

 
TMGQLYEVMVLVAQYWMPSSAVWHPLALVLDALITHLRSGAEGVIYPDPL
TYGSVRL
  
LTVGQFYFLIRKRIHLRPEDALFFF
 

Matching transcripts

 GABARAPL1-002 - ENSP00000411256 [100%]
GABARAPL1-018 - ENSP00000445857 [100%]
  GABARAPL1-001 - ENSP00000266458 [100%]
GABARAPL1-002 - ENSP00000411256 [100%]
GABARAPL1-013 - ENSP00000444186 [100%]
GABARAPL1-018 - ENSP00000445857 [100%]
 

Other gene match

   GABARAP - ENSG00000170296 [96%]
  
ANTIGEN VIEW
GABARAPL1-001
GABARAPL1-002
GABARAPL1-013
GABARAPL1-018
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.