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SYCP3

ANTIBODY INFORMATION
 
 

Antibody HPA039635

  

Antibody HPA043938

  

Antibody CAB032978

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA039635   HPA043938   sc-33195  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   12   7  

References

      

Proper citation

 Atlas Antibodies Cat#HPA039635, RRID:AB_2676607   Atlas Antibodies Cat#HPA043938, RRID:AB_2678740   Santa Cruz Biotechnology Cat#sc-33195, RRID:AB_656732  

VALIDATION SUMMARY
 
 

Antibody HPA039635

  

Antibody HPA043938

  

Antibody CAB032978

  
Immunohistochemistry


Validated

Validated
  

Validated

Validated
   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039635

  

Antibody HPA043938

  

Antibody CAB032978

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

 
   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.    
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA043938 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA039635 across 74 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.   Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.   Immunohistochemical staining of human testis shows moderate nuclear positivity in cells of seminiferus ducts.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 2 cell types		   RNA: detected in 1 tissues
Protein: detected in 3 cell types		   RNA: detected in 1 tissues
Protein: detected in 31 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:1700   1:2000   1:200  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA039635

  

Antibody HPA043938

  

Antibody CAB032978

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 27.7   27.7   27.7  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA039635

  

Antibody HPA043938

  

Antibody CAB032978

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:12000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA039635

  

Antibody HPA043938

  

Antibody CAB032978

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 87   71    

Antigen sequence

 
ILNMFRQQQKILQQSRIVQSQRLKTIKQLYEQFIKSMEELEKNHDNLLTG
AQNEFKKEMAMLQKKIMMETQQQEIASVRKSLQSMLF
  
KYSRKSGKPSVEDQFTRAYDFETEDKKDLSGSEEDVIEGKTAVIEKRRKK
RSSAGVVEDMGGEVQNMLEGV
  

											 
																				

																		

										
											
Matching transcripts

												
																					SYCP3-001 - ENSP00000376658 [100%] 
SYCP3-002 - ENSP00000266743 [100%] 
SYCP3-003 - ENSP00000376655 [100%] 

											 
																						SYCP3-001 - ENSP00000376658 [100%] 
SYCP3-002 - ENSP00000266743 [100%] 
SYCP3-003 - ENSP00000376655 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SYCP3-001
SYCP3-002
SYCP3-003
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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