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Antibody staining overlaps with antibody HPA040787.
Validated
Antibody staining overlaps with antibody HPA039404.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane & cytosol.
Antibody dilution
1:200
1:35
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA039404
Antibody HPA040787
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA040787 across 70 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA039404 across 70 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human breast shows strong cytoplasmic and membrane positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 68 cell types
RNA: detected in 37 tissues Protein: detected in 41 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:150
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039404
Antibody HPA040787
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408581)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408581)
Target mass (kDa)
134.1
134.1
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA039404
Antibody HPA040787
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
