RLBP1 - Antibodies - The Human Protein Atlas

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RLBP1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA042051

Antibody HPA044083

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA042051

HPA044083

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA044083, RRID:AB_2678802

VALIDATION SUMMARY

Antibody HPA042051

Antibody HPA044083

Immunocytochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA042051

Antibody HPA044083

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm, cytosol & centrosome.

Antibody dilution

1:105

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA042051

Antibody HPA044083

Standard validation

Approved

Figure description

Positivity observed in microglia, in all brain regions.

WESTERN BLOT

Antibody HPA042051

Antibody HPA044083

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.

Figure description

Target mass (kDa)

36.5

36.5, 12.1

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA042051

Antibody HPA044083

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:12000

ANTIGEN INFORMATION

Antibody HPA042051

Antibody HPA044083

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

TTKDHGPVFGPCSQLPRHTLQKAKDELNEREETREEAVRELQEMVQAQAA
SGEELAVAVAERVQEKDSGFFLRFIRARKFNVGRAYELLRGYVNFRL

PARFKAIHFIHQPWYFTTTYNVVKPFLKSKLLERVFVHGDDLSGFYQEID
ENILPSD

Matching transcripts

RLBP1-001 - ENSP00000268125 [100%]

RLBP1-001 - ENSP00000268125 [100%]
RLBP1-005 - ENSP00000454740 [81%]

Other gene match

ANTIGEN VIEW

RLBP1-001	RLBP1-005

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.