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PDPK1

ANTIBODY INFORMATION
 
 

Antibody HPA035199

  

Antibody HPA068961

  

Antibody CAB004272

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA035199   HPA068961   sc-17765  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   16   2  

References

      

Proper citation

 Atlas Antibodies Cat#HPA035199, RRID:AB_2674514   Atlas Antibodies Cat#HPA068961, RRID:AB_2686063   Santa Cruz Biotechnology Cat#sc-17765, RRID:AB_626657  

VALIDATION SUMMARY
 
 

Antibody HPA035199

  

Antibody HPA068961

  

Antibody CAB004272

  
Immunocytochemistry

  Approved    
Immunohistochemistry

Supported     Supported  
Western blot

Uncertain   Supported   Supported  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035199

  

Antibody HPA068961

  

Antibody CAB004272

  

Standard validation

   Approved    

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.    

Antibody dilution

   1:200    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035199

  

Antibody HPA068961

  

Antibody CAB004272

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.     Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 54 cell types		     RNA: detected in 37 tissues
Protein: detected in 65 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:2500     1:6000  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA035199

  

Antibody HPA068961

  

Antibody CAB004272

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 63.2, 60.3, 48.2   63.2, 60.3, 48.2   63.2, 60.3, 50.8, 48.2, 16.8, 10.3  

Antibody dilution

 1:250   1:860   1:500  

PROTEIN ARRAY
 
 

Antibody HPA035199

  

Antibody HPA068961

  

Antibody CAB004272

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:10250    

ANTIGEN INFORMATION
 
 

Antibody HPA035199

  

Antibody HPA068961

  

Antibody CAB004272

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 74   83    

Antigen sequence

 
HSLSASDTGLPQRSGSNIEQYIHDLDSNSFELDLQFSEDEKRLLLEKQAG
GNPWHQFVENNLILKMGPVDKRKG
  
LFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKNFKTFFVHT
PNRTYYLMDPSGNAHKWCRKIQEVWRQRYQSHP
  

											 
																				

																		

										
											
Matching transcripts

												
																					PDPK1-001 - ENSP00000344220 [100%] 
PDPK1-002 - ENSP00000268673 [100%] 
PDPK1-003 - ENSP00000373876 [100%] 

											 
																						PDPK1-001 - ENSP00000344220 [100%] 
PDPK1-002 - ENSP00000268673 [100%] 
PDPK1-003 - ENSP00000373876 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

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PDPK1-001
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