CLTC - Antibodies - The Human Protein Atlas

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CLTC

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA059143

Antibody CAB010389

Antibody CAB011571

Antibody CAB017155

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Abcam plc

Kazusa DNA Research Institute

Product name

HPA059143

sc-9069

ab21679

GX0111

Host species

Rabbit

Clonality

pAb

msAb

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Affinity

Not known

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA059143, RRID:AB_2683924

Santa Cruz Biotechnology Cat#sc-9069, RRID:AB_2083184

Abcam Cat#ab21679, RRID:AB_2083165

VALIDATION SUMMARY

Antibody HPA059143

Antibody CAB010389

Antibody CAB011571

Antibody CAB017155

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA059143

Antibody CAB010389

Antibody CAB011571

Antibody CAB017155

Formal validation: Tagged protein

Validated

Antibody staining overlaps with GFP tagged protein

Figure description

Immunofluorescent staining of transgenic HeLa cells show antibody staining in endosome & lysosome and GFP expression in endosome & lysosome.

Antibody dilution

1:60

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to endosomes & lysosomes.

Immunofluorescent staining of human cell line A-431 shows localization to vesicles.

Antibody dilution

1:60

1:200

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA059143

Antibody CAB010389

Antibody CAB011571

Antibody CAB017155

Standard validation

Approved

Figure description

Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human testis shows cytoplasmic positivity in cells of seminiferus ducts.

Immunohistochemical staining of human epididymis shows distinct cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 67 cell types

RNA: detected in 37 tissues
Protein: detected in 66 cell types

RNA: detected in 37 tissues
Protein: detected in 50 cell types

RNA: detected in 37 tissues
Protein: detected in 67 cell types

Retrieval

HIER pH6

Antibody dilution

1:900

1:50

1:7000

1:1000

Literature conformity

Consistent with extensive gene/protein characterization data.

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA059143

Antibody CAB010389

Antibody CAB011571

Antibody CAB017155

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 213, 118, 83, 50, 33, 26, 18
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

192.1, 191.6, 70.7, 25.7

192.1, 191.6, 187.9, 70.7, 25.7, 15.5, 13.7

Antibody dilution

1:200

1:500

PROTEIN ARRAY

Antibody HPA059143

Antibody CAB010389

Antibody CAB011571

Antibody CAB017155

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2400

ANTIGEN INFORMATION

Antibody HPA059143

Antibody CAB010389

Antibody CAB011571

Antibody CAB017155

Antigen

Recombinant protein fragment

Recombinant protein

Synthetic peptide

Recombinant protein

Length (aa)

Antigen sequence

ESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQ

Matching transcripts

CLTC-001 - ENSP00000269122 [100%]
CLTC-008 - ENSP00000465200 [100%]
CLTC-010 - ENSP00000462252 [100%]
CLTC-201 - ENSP00000479606 [100%]

Other gene match

ANTIGEN VIEW

CLTC-001	CLTC-008	CLTC-010	CLTC-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.