GRB7 - Antibodies - The Human Protein Atlas

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GRB7

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA057084

Antibody CAB005226

Antibody CAB073538

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Monash University MATF

Product name

HPA057084

sc-606

HUPO 11 (anti-GRB7)

Host species

Rabbit

Mouse

Clonality

pAb

msAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA057084, RRID:AB_2683330

Santa Cruz Biotechnology Cat#sc-606, RRID:AB_2113278

VALIDATION SUMMARY

Antibody HPA057084

Antibody CAB005226

Antibody CAB073538

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA057084

Antibody CAB005226

Antibody CAB073538

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane & cytosol.

Antibody dilution

1:200

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA057084

Antibody CAB005226

Antibody CAB073538

Standard validation

Approved

Figure description

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Immunohistochemical staining of human pancreas shows cytoplasmic positivity in exocrine glandular cells.

Immunohistochemical staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.

Expression

RNA: detected in 27 tissues
Protein: detected in 22 cell types

RNA: detected in 27 tissues
Protein: detected in 46 cell types

RNA: detected in 27 tissues
Protein: detected in 56 cell types

Retrieval

HIER pH6

Antibody dilution

1:1000

1:15

1:22000

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA057084

Antibody CAB005226

Antibody CAB073538

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

62.1

62.1, 59.7, 49.4, 12.6

Antibody dilution

1:390

1:500

1:1000

PROTEIN ARRAY

Antibody HPA057084

Antibody CAB005226

Antibody CAB073538

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:4650

ANTIGEN INFORMATION

Antibody HPA057084

Antibody CAB005226

Antibody CAB073538

Antigen

Recombinant protein fragment

Synthetic peptide

Not known

Length (aa)

Antigen sequence

QGHTTGSVKPLSRSDAMELDLSPPHLSSSPEDLCPAPGTPPGTPRPPDTP
LPEEVKRSQPLLIPTTGRKL

Matching transcripts

GRB7-006 - ENSP00000403459 [99%]

Other gene match

ANTIGEN VIEW

GRB7-006

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.