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SOD1

ANTIBODY INFORMATION
 
 

Antibody HPA001401

  

Antibody CAB008670

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   AbFrontier
  

Product name

 HPA001401   LF-PA0013  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Ammonium Sulfate Precipitation  

Other gene match

    

Released in version

 2   3  

References

 2    

Proper citation

 Atlas Antibodies Cat#HPA001401, RRID:AB_1080132   ABFrontier Cat#LF-PA0013, RRID:AB_1623312  

VALIDATION SUMMARY
 
 

Antibody HPA001401

  

Antibody CAB008670

  
Immunocytochemistry


Validated
    
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001401

  

Antibody CAB008670

  

Formal validation: Tagged protein
Validated

Antibody staining overlaps with GFP tagged protein		    

   

Figure description

 Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleus, plasma membrane & cytoplasm and GFP expression in nucleus, plasma membrane & cytoplasm.    

Antibody dilution

 1:10    
 

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.    

Antibody dilution

 1:10    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001401

  

Antibody CAB008670

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human liver shows nuclear and cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in glial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:600  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001401

  

Antibody CAB008670

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 15.9, 13.9   15.9, 13.9  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001401

  

Antibody CAB008670

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1000    

ANTIGEN INFORMATION
 
 

Antibody HPA001401

  

Antibody CAB008670

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 135    

Antigen sequence

 
PVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGP
HFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCI
IGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACG
  

											 
																				

																		

										
											
Matching transcripts

												
																					SOD1-001 - ENSP00000270142 [100%] 
SOD1-002 - ENSP00000374645 [92%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SOD1-001
SOD1-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001401

												
										 
																				
											
											
											
Antibody CAB008670

												
										 
																		

																	

										
											
1.

												
																						NQO2 is a reactive oxygen species generating off-target for acetaminophen

	Miettinen TP et al

	Mol Pharm 2014;11(12):4395-404

			Application: WB				
											 
																						
											 
																				

																		

										
											
2.

												
																						Marinesco-Sjögren syndrome protein SIL1 regulates motor neuron subtype-selective ER stress in ALS

	Filézac de L'Etang A et al

	Nat Neurosci 2015;18(2):227-38

			Application: IHC, WB				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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