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MMEL1

ANTIBODY INFORMATION
 
 

Antibody HPA007876

  

Antibody HPA008205

  

Antibody CAB020786

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA007876   HPA008205   3215.00.02  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 4   4   5  

References

 1      

Proper citation

 Atlas Antibodies Cat#HPA007876, RRID:AB_1854012   Atlas Antibodies Cat#HPA008205, RRID:AB_1854011    

VALIDATION SUMMARY
 
 

Antibody HPA007876

  

Antibody HPA008205

  

Antibody CAB020786

  
Immunohistochemistry

Uncertain   Uncertain   Uncertain  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007876

  

Antibody HPA008205

  

Antibody CAB020786

  

Standard validation

 Uncertain   Uncertain   Uncertain  

     

Figure description

 Immunohistochemical staining of human testis shows moderate positivity in seminiferus duct cells.   Immunohistochemical staining of human testis shows moderate positivity in seminiferus duct cells.   Immunohistochemical staining of human testis shows moderate positivity in seminiferus duct cells.  

Expression

 RNA: detected in 4 tissues
Protein: detected in 71 cell types		   RNA: detected in 4 tissues
Protein: detected in 62 cell types		   RNA: detected in 4 tissues
Protein: detected in 43 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:25   1:1000  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007876

  

Antibody HPA008205

  

Antibody CAB020786

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 89.4, 71.1   89.4   89.4, 71.1, 7.5  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA007876

  

Antibody HPA008205

  

Antibody CAB020786

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA007876

  

Antibody HPA008205

  

Antibody CAB020786

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Genetic immunization  

Length (aa)

 148   133    

Antigen sequence

 
LEELQSQFGLKGFNWTLFIQTVLSSVKIKLLPDEEVVVYGIPYLQNLENI
IDTYSARTIQNYLVWRLVLDRIGSLSQRFKDTRVNYRKALFGTMVEEVRW
RECVGYVNSNMENAVGSLYVREAFPGDSKSMVRELIDKVRTVFVETLD
  
SVIEKRGSQPLLDILEVVGGWPVAMDRWNETVGLEWELERQLALMNSQFN
RRVLIDLFIWNDDQNSSRHIIYIDQPTLGMPSREYYFNGGSNRKVREAYL
QFMVSVATLLREDANLPRDSCLVQEDMVQVLEL
  

											 
																				

																		

										
											
Matching transcripts

												
																					MMEL1-001 - ENSP00000367668 [100%] 
MMEL1-006 - ENSP00000422492 [100%] 

											 
																						MMEL1-001 - ENSP00000367668 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MMEL1-001
MMEL1-006
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA007876

												
										 
																				
											
											
											
Antibody HPA008205

												
										 
																				
											
											
											
Antibody CAB020786

												
										 
																		

																	

										
											
1.

												
																						Autocrine regulation of human sperm motility by tachykinins

	Pinto FM et al

	Reprod Biol Endocrinol 2010;8:104

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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