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Immunofluorescent staining of human cell line CACO-2 shows localization to nucleus.
Antibody dilution
1:106
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA015576
Antibody HPA072047
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human smooth muscle shows moderate cytoplasmic positivity in smooth muscle cells.
Expression
RNA: detected in 33 tissues Protein: detected in 78 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA015576
Antibody HPA072047
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
60.7, 60.4, 58.6, 49.7, 46, 43.9
60.7, 60.4, 58.6, 49.7, 46, 43.9
Antibody dilution
1:250
1:350
PROTEIN ARRAY
Antibody HPA015576
Antibody HPA072047
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
