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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:60
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA001821
Standard validation
Approved
Figure description
RGS5 is mainly located in the synapse. In a subset of neurons RGS5 is located in the soma.
WESTERN BLOT
Antibody HPA001821
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418548)
Target mass (kDa)
23.6, 21.4, 20.9
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA001821
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
