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Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
Antibody dilution
1:20
1:50
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA019463
Antibody HPA019481
Antibody CAB017151
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA019481 across 43 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA019463 across 43 cell lines.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human gallbladder shows nuclear positivity in glandular cells.
Immunohistochemical staining of human gall bladder shows strong nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 64 cell types
RNA: detected in 37 tissues Protein: detected in 73 cell types
RNA: detected in 37 tissues Protein: detected in 78 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:60
1:40
1:200
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019463
Antibody HPA019481
Antibody CAB017151
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416956)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416956)
Target mass (kDa)
52.4, 28.9, 22.5
52.4
52.4, 28.9, 22.5
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA019463
Antibody HPA019481
Antibody CAB017151
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
