TUFT1 - Antibodies - The Human Protein Atlas

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TUFT1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA028112

Antibody HPA054301

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA028112

HPA054301

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA028112, RRID:AB_10603224

Atlas Antibodies Cat#HPA054301, RRID:AB_2682442

VALIDATION SUMMARY

Antibody HPA028112

Antibody HPA054301

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA028112

Antibody HPA054301

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to vesicles.

Antibody dilution

1:41

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA028112

Antibody HPA054301

Standard validation

Approved

Figure description

Immunohistochemical staining of human skin shows moderate positivity in a subset of keratinocytes.

Immunohistochemical staining of human skin shows cytoplasmic positivity in squamous epithelial cells.

Expression

RNA: detected in 36 tissues
Protein: detected in 55 cell types

RNA: detected in 36 tissues
Protein: detected in 56 cell types

Retrieval

HIER pH6

Antibody dilution

1:500

1:75

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA028112

Antibody HPA054301

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

44.3, 41.5, 38

44.3, 41.5, 22.6

Antibody dilution

1:250

1:210

PROTEIN ARRAY

Antibody HPA028112

Antibody HPA054301

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:1650

ANTIGEN INFORMATION

Antibody HPA028112

Antibody HPA054301

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

DCVAFEVTLSRYQREAEQSNVALQREEDRVEQKEAEVGELQRRLLGMETE
HQALLAKVREGEVALEELRSNNADCQAEREKAATLEKEVAGLREKIHHL

KSEVQYIQEARNCLQKLREDISSKLDRNLGDSLHRQEIQVVLEKPNGFSQ
SPTALYSSPPEVDTCINEDVESLRKTVQDL

Matching transcripts

TUFT1-006 - ENSP00000357842 [100%]
TUFT1-008 - ENSP00000357841 [100%]
TUFT1-010 - ENSP00000376476 [100%]

TUFT1-006 - ENSP00000357842 [100%]
TUFT1-008 - ENSP00000357841 [100%]
TUFT1-201 - ENSP00000343781 [100%]

Other gene match

ANTIGEN VIEW

TUFT1-006	TUFT1-008	TUFT1-010	TUFT1-201

RELEVANT PUBLICATIONS

Antibody HPA028112

Antibody HPA054301

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.