Fields »
ABOUT
HELP
BLOG
ANP32E

ANTIBODY INFORMATION
 
 

Antibody HPA063133

  

Antibody HPA067561

  

Antibody CAB021114

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   GenWay Biotech
  

Product name

 HPA063133   HPA067561   15-288-21207  

Host species

 Rabbit   Rabbit   Hen  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

 ANP32B - ENSG00000136938 [84%]
ANP32D - ENSG00000139223 [96%]
ANP32A - ENSG00000140350 [100%]
   ANP32B - ENSG00000136938 [81%]
ANP32D - ENSG00000139223 [97%]
ANP32A - ENSG00000140350 [100%]
    

Released in version

 14   14   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA063133, RRID:AB_2684947   Atlas Antibodies Cat#HPA067561, RRID:AB_2685861   GenWay Biotech Inc. Cat#15-288-21207, RRID:AB_10057407  

VALIDATION SUMMARY
 
 

Antibody HPA063133

  

Antibody HPA067561

  

Antibody CAB021114

  
Immunocytochemistry

Approved   Approved    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA063133

  

Antibody HPA067561

  

Antibody CAB021114

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm, cytosol & centrosome.   Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & centrosome.    

Antibody dilution

 1:8   1:46    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA063133

  

Antibody HPA067561

  

Antibody CAB021114

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.   Immunohistochemical staining of human testis shows distinct nuclear and cytoplasmic positivity in cells of the seminiferus ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 80 cell types		   RNA: detected in 37 tissues
Protein: detected in 77 cell types		   RNA: detected in 37 tissues
Protein: detected in 51 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:900   1:5000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA063133

  

Antibody HPA067561

  

Antibody CAB021114

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 30.7, 28.8, 28.6, 25.1, 20, 19.6, 16.9, 14.8, 13.1   30.7, 28.8, 28.6, 25.1, 20, 19.6, 16.9, 14.8, 13.1   30.7, 26.3, 25.1, 19.6, 16.9, 15.9, 13.1, 12.2, 11.8  

Antibody dilution

 1:80   1:150   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA063133

  

Antibody HPA067561

  

Antibody CAB021114

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:1850    

ANTIGEN INFORMATION
 
 

Antibody HPA063133

  

Antibody HPA067561

  

Antibody CAB021114

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 25   32    

Antigen sequence

 
KCPNLTHLNLSGNKIKDLSTIEPLK
  
GLEVLAEKCPNLTHLNLSGNKIKDLSTIEPLK
  

											 
																				

																		

										
											
Matching transcripts

												
																					ANP32E-001 - ENSP00000463154 [84%] 
ANP32E-002 - ENSP00000481415 [84%] 
ANP32E-004 - ENSP00000358110 [84%] 
ANP32E-006 - ENSP00000435215 [84%] 
ANP32E-007 - ENSP00000432684 [84%] 

											 
																						ANP32E-001 - ENSP00000463154 [88%] 
ANP32E-002 - ENSP00000481415 [88%] 
ANP32E-004 - ENSP00000358110 [88%] 
ANP32E-006 - ENSP00000435215 [88%] 
ANP32E-007 - ENSP00000432684 [88%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					ANP32B - ENSG00000136938 [84%]
ANP32D - ENSG00000139223 [96%]
ANP32A - ENSG00000140350 [100%]

											 
																						ANP32B - ENSG00000136938 [81%]
ANP32D - ENSG00000139223 [97%]
ANP32A - ENSG00000140350 [100%]

											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ANP32E-001
ANP32E-002
ANP32E-004
ANP32E-006
ANP32E-007
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.