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Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Antibody dilution
1:80
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA028404
Antibody HPA028698
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA028698 across 45 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA028404 across 45 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA028698 across 75 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA028404 across 75 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human duodenum shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human vulva/anal skin shows strong positivity in stratum granulosum.
Expression
RNA: detected in 37 tissues Protein: detected in 45 cell types
RNA: detected in 37 tissues Protein: detected in 48 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:800
1:400
Literature conformity
Partly consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA028404
Antibody HPA028698
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418577)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
38.4
38.4
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA028404
Antibody HPA028698
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
