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TAF1A

ANTIBODY INFORMATION
 
 

Antibody HPA053101

  

Antibody HPA054334

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA053101   HPA054334  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   11  

References

    

Proper citation

 Atlas Antibodies Cat#HPA053101, RRID:AB_2682045   Atlas Antibodies Cat#HPA054334, RRID:AB_2682453  

VALIDATION SUMMARY
 
 

Antibody HPA053101

  

Antibody HPA054334

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

  Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA053101

  

Antibody HPA054334

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleus & vesicles.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & microtubules.  

Antibody dilution

 1:25   1:139  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA053101

  

Antibody HPA054334

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.  

Expression

   RNA: detected in 33 tissues
Protein: detected in 69 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:600  

Literature conformity

   Partly consistent with gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA053101

  

Antibody HPA054334

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 52.7, 39.6   52.7  

Antibody dilution

 1:120   1:690  

PROTEIN ARRAY
 
 

Antibody HPA053101

  

Antibody HPA054334

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:5550  

ANTIGEN INFORMATION
 
 

Antibody HPA053101

  

Antibody HPA054334

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 92   92  

Antigen sequence

 
ILMGNHLAWVQEEWNSRKNWWPGFHFSYFWAKSDWKEDTALACEKAFVAG
LLLGKGCRYFRYILKQDHQILGKKIKRMKRSVKKYSIVNPRL
  
HQWQQAAEYMYSYFQTLEDSDSYKRQAAPEIIWKLGSEILFYHPKSNMES
FNTFANRMKNIGVMNYLKISLQHALYLLHHGMLKDAKRNLSE
 

Matching transcripts

 TAF1A-001 - ENSP00000327072 [100%]
TAF1A-002 - ENSP00000355856 [100%]
TAF1A-201 - ENSP00000339976 [100%]
  TAF1A-001 - ENSP00000327072 [100%]
TAF1A-201 - ENSP00000339976 [100%]
 

Other gene match

    
ANTIGEN VIEW
TAF1A-001
TAF1A-002
TAF1A-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.