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ANTIBODY INFORMATION
 
 

Antibody HPA024343

  

Antibody CAB026182

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA024343   AF3607  

Host species

 Rabbit   Goat  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 5   5  

References

    

Proper citation

 Atlas Antibodies Cat#HPA024343, RRID:AB_1847162    

VALIDATION SUMMARY
 
 

Antibody HPA024343

  

Antibody CAB026182

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Supported  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024343

  

Antibody CAB026182

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human oral mucosa shows strong cytoplasmic positivity in squamous epithelial cells.   Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in superficial squamous epithelial cells.  

Expression

 RNA: detected in 6 tissues
Protein: detected in 12 cell types		   RNA: detected in 6 tissues
Protein: detected in 16 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:120   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA024343

  

Antibody CAB026182

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 53.5   53.5  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA024343

  

Antibody CAB026182

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA024343

  

Antibody CAB026182

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 100    

Antigen sequence

 
YDRQAESQSQERISPQIQLSGQTEQTQKAGEGKRNQTTEMRPERQPQTRE
QDRAHQTGETVTGSGTQTQAGATQTVEQDSSHQTGRTSKQTQEATNDQNR
  

											 
																				

																		

										
											
Matching transcripts

												
																					CRNN-001 - ENSP00000271835 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CRNN-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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