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NUP210L

ANTIBODY INFORMATION
 
 

Antibody HPA047195

  

Antibody HPA064245

  

Antibody HPA066707

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA047195   HPA064245   HPA066707  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 16   14   14  

References

      

Proper citation

 Atlas Antibodies Cat#HPA047195, RRID:AB_2679980   Atlas Antibodies Cat#HPA064245, RRID:AB_2685224   Atlas Antibodies Cat#HPA066707, RRID:AB_2685706  

VALIDATION SUMMARY
 
 

Antibody HPA047195

  

Antibody HPA064245

  

Antibody HPA066707

  
Immunocytochemistry

Approved      
Immunohistochemistry

  Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA047195

  

Antibody HPA064245

  

Antibody HPA066707

  

Standard validation

 Approved      

     

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nuclear bodies & cytosol.      

Antibody dilution

 1:7      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047195

  

Antibody HPA064245

  

Antibody HPA066707

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts.  

Expression

   RNA: detected in 1 tissues
Protein: detected in 35 cell types		   RNA: detected in 1 tissues
Protein: detected in 38 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:5500   1:3000  

Literature conformity

   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA047195

  

Antibody HPA064245

  

Antibody HPA066707

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 210.6, 194, 73.9   210.6, 194   210.6  

Antibody dilution

 1:80   1:880   1:220  

PROTEIN ARRAY
 
 

Antibody HPA047195

  

Antibody HPA064245

  

Antibody HPA066707

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:600   1:10600   1:2600  

ANTIGEN INFORMATION
 
 

Antibody HPA047195

  

Antibody HPA064245

  

Antibody HPA066707

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 128   84   82  

Antigen sequence

 
PINSQLKLHTNREGAAFVSSRVLKCFPNSSVIEEDGEGLLKAGSIAGTAV
LEVTSIEPFGVNQTTITGVQVAPVTYLRVSSQPKLYTAQGRTLSAFPLGM
SLTFTVQFYNSIGEKFHTHNTQLYLALN
  
VQNPFRYGEIKIHVLKLNKMELLPFHADVEIGQIIEIPIAMYHINKETKE
AMAFTDCSHLSLDLNMDKQGVFTLLKEGIQRPGP
  
YVCIIKVRPQSEELLQALSVADTSVYGWATLVSERSKNGMQRILIPFIPA
FYINQSELVLSHKQDIGEIRVLGVDRVLRKLE
 

Matching transcripts

 NUP210L-001 - ENSP00000357547 [100%]
NUP210L-002 - ENSP00000357541 [100%]
NUP210L-201 - ENSP00000271854 [100%]
  NUP210L-001 - ENSP00000357547 [100%]
NUP210L-201 - ENSP00000271854 [100%]
  NUP210L-001 - ENSP00000357547 [100%]
 

Other gene match

      
ANTIGEN VIEW
NUP210L-001
NUP210L-002
NUP210L-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.