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Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
Antibody dilution
1:74
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA017990
Antibody HPA057127
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.
Expression
RNA: detected in 26 tissues Protein: detected in 41 cell types
Retrieval
HIER pH6
Antibody dilution
1:800
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA017990
Antibody HPA057127
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406879)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
82.8, 81.4, 48, 47.1
82.8, 81.4
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA017990
Antibody HPA057127
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:60000
1:2950
ANTIGEN INFORMATION
Antibody HPA017990
Antibody HPA057127
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
102
66
Antigen sequence
VDLSKMQNVMYDLITELNDRSEDLEKQIGSLESKLEHLTASFNSLPLLIA
DTLRQQQQQLLSAIIEARGVSVAVGTTHTPISDSPIGVSSTSFPTPYTSS
SS
