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CEP170

ANTIBODY INFORMATION
 
 

Antibody HPA042151

  

Antibody HPA045787

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042151   HPA045787  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   12  

References

    

Proper citation

 Atlas Antibodies Cat#HPA042151, RRID:AB_2677876   Atlas Antibodies Cat#HPA045787, RRID:AB_2679453  

VALIDATION SUMMARY
 
 

Antibody HPA042151

  

Antibody HPA045787

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA042151

  

Antibody HPA045787

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA045787.		  
Validated

Antibody staining overlaps with antibody HPA042151.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & centrosome.   Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane, cytosol & centrosome.  

Antibody dilution

 1:143   1:200  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042151

  

Antibody HPA045787

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human bronchus shows moderate cytoplasmic positivity in respiratory epithelial cells.   Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 29 cell types		   RNA: detected in 36 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1000   1:1200  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042151

  

Antibody HPA045787

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 175.3, 172, 164.5, 161.4, 35.5, 32.7, 29.1, 19.9   175.3, 172, 164.5, 161.4  

Antibody dilution

 1:250   1:1360  

PROTEIN ARRAY
 
 

Antibody HPA042151

  

Antibody HPA045787

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:10900  

ANTIGEN INFORMATION
 
 

Antibody HPA042151

  

Antibody HPA045787

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 101   99  

Antigen sequence

 
IPPLVHSKTPEGNNGRSGDPRPQAAEPPDHLTITRRRTWSRDEVMGDNLL
LSSVFQFSKKIRQSIDKTAGKIRILFKDKDRNWDDIESKLRAESEVPIVK
T
  
SQWASLAANHTRHDQEERIMEFSAPLPLENETEISESGMTVRSTGSATSL
ASQGERRRRTLPQLPNEEKSLESHRAKVVTQRSEIGEKQDTELQEKETP
 

Matching transcripts

 CEP170-001 - ENSP00000355502 [100%]
CEP170-002 - ENSP00000355501 [100%]
CEP170-003 - ENSP00000355500 [100%]
CEP170-004 - ENSP00000338161 [100%]
CEP170-006 - ENSP00000430142 [100%]
CEP170-007 - ENSP00000427842 [100%]
CEP170-026 - ENSP00000394002 [100%]
CEP170-009 - ENSP00000427843 [98%]
  CEP170-001 - ENSP00000355502 [100%]
CEP170-002 - ENSP00000355501 [100%]
CEP170-003 - ENSP00000355500 [100%]
CEP170-004 - ENSP00000338161 [100%]
 

Other gene match

    
ANTIGEN VIEW
CEP170-001
CEP170-002
CEP170-003
CEP170-004
CEP170-006
CEP170-007
CEP170-009
CEP170-026
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.