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PARP1

ANTIBODY INFORMATION
 
 

Antibody HPA045168

  

Antibody CAB000147

  

Antibody CAB003839

  

Antibody CAB003840

  

Antibody CAB075726

  

Antibody CAB075727

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Promega
   Epitomics
   Epitomics
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA045168   G7341   1051-1   1072-1   AMAb90959   AMAb90960  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known   Supernatant   Supernatant   Protein A/G   Protein A/G  

Other gene match

            

Released in version

 10   1   2   2   14   14  

References

            

Proper citation

 Atlas Antibodies Cat#HPA045168, RRID:AB_2679240   Promega Cat#G7341, RRID:AB_430876       Atlas Antibodies Cat#AMAb90959, RRID:AB_2665732   Atlas Antibodies Cat#AMAb90960, RRID:AB_2665733  

VALIDATION SUMMARY
 
 

Antibody HPA045168

  

Antibody CAB000147

  

Antibody CAB003839

  

Antibody CAB003840

  

Antibody CAB075726

  

Antibody CAB075727

  
Immunocytochemistry


Validated
     Supported   Supported      
Immunohistochemistry

Supported   Supported   Supported  

Validated
   Supported   Supported  
Western blot


Validated
   Supported   Uncertain   Uncertain   Supported   Supported  
Protein array

Supported            

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA045168

  

Antibody CAB000147

  

Antibody CAB003839

  

Antibody CAB003840

  

Antibody CAB075726

  

Antibody CAB075727

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		            

           

Figure description

 Nuclear region of segmented cells in 10x-images            

Antibody dilution

 1:81            
 

Standard validation

 Supported     Supported   Supported      

           

Figure description

 Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus.     Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleus.      

Antibody dilution

 1:81     1:25   1:6      

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.   The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045168

  

Antibody CAB000147

  

Antibody CAB003839

  

Antibody CAB003840

  

Antibody CAB075726

  

Antibody CAB075727

  

Formal validation: Orthogonal

      
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		      

     
     

Figure description

       Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.      
 

Standard validation

 Supported   Supported   Supported   Supported   Supported   Supported  

           

Figure description

 Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.   Immunohistochemical staining of human placenta shows moderate nuclear positivity in trophoblastic cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity, with a granular pattern, in exocrine glandular cells.   Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells, cells in molecular layer   Immunohistochemical staining of human tonsil shows strong nuclear positivity in germinal center cells and non-germinal center cells.   Immunohistochemical staining of human tonsil shows strong positivity in germinal center cells and non-germinal center cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		   RNA: detected in 37 tissues
Protein: detected in 12 cell types		   RNA: detected in 37 tissues
Protein: detected in 19 cell types		   RNA: detected in 37 tissues
Protein: detected in 68 cell types		   RNA: detected in 37 tissues
Protein: detected in 78 cell types		   RNA: detected in 37 tissues
Protein: detected in 79 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:1200   1:200   1:100   1:25   1:1500   1:40000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA045168

  

Antibody CAB000147

  

Antibody CAB003839

  

Antibody CAB003840

  

Antibody CAB075726

  

Antibody CAB075727

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		            

           

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		            

Target mass (kDa)

 113.1            

Loading control

 Total protein image            

Antibody dilution

 1:180            
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

           

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		       Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 113.1   113.1, 17.3, 12.2   113.1, 17.3, 12.2   113.1, 17.3, 12.2   113.1, 17.3, 12.2   113.1, 17.3, 12.2  

Antibody dilution

 1:250   1:500   1:500   1:500   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA045168

  

Antibody CAB000147

  

Antibody CAB003839

  

Antibody CAB003840

  

Antibody CAB075726

  

Antibody CAB075727

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		            

           

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.            

Antibody dilution

 1:3000            

ANTIGEN INFORMATION
 
 

Antibody HPA045168

  

Antibody CAB000147

  

Antibody CAB003839

  

Antibody CAB003840

  

Antibody CAB075726

  

Antibody CAB075727

  

Antigen

 Recombinant protein fragment   Not known   Synthetic peptide   Synthetic peptide   Recombinant protein   Recombinant protein  

Length (aa)

 139            

Antigen sequence

 
KGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIG
SNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQ
DEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAM
  

											 
																						

											 
																						

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					PARP1-001 - ENSP00000355759 [100%] 

											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PARP1-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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